人多發(fā)性骨髓瘤細胞OPM2

細胞名稱

人多發(fā)性骨髓瘤細胞OPM2

貨物編碼

BFN60808728

產(chǎn)品規(guī)格

T25培養(yǎng)瓶x1

1.5ml凍存管x2

細胞數(shù)量

1x10^6

1x10^6

保存溫度

37℃

-198℃

運輸方式

常溫保溫運輸

干冰運輸

安全等級

1

用途限制

僅供科研     2類

培養(yǎng)體系

DMEM高糖+10%FBS+1%三抗

培養(yǎng)溫度

37℃

二氧化碳濃度

5%

簡介

人多發(fā)性骨髓瘤細胞OPM2取自56歲女性供體。該細胞源于DSMZ。

注釋

Part of: Cancer Cell Line Encyclopedia (CCLE) project.

Part of: COSMIC cell lines project.

Part of: LL-100 blood cancer cell line panel.

Part of: MD Anderson Cell Lines Project.

Characteristics: Produces Ig lambda.

Doubling time: 30-36 hours (PubMed=3926660); 50 hours (PubMed=25984343); ~50-60 hours (DSMZ).

Microsatellite instability: Stable (MSS) (Sanger).

Omics: Array-based CGH.

Omics: Deep exome analysis.

Omics: Deep quantitative proteome analysis.

Omics: Deep RNAseq analysis.

Omics: DNA methylation analysis.

Omics: Protein expression by reverse-phase protein arrays.

Omics: shRNA library screening.

Omics: SNP array analysis.

Omics: Transcriptome analysis.

Derived from sampling site: Peripheral blood.

基因突變

Heterozygous for CDKN2A p.His83Tyr (c.247C>T) (Cosmic-CLP).

Heterozygous for FGFR3 p.Lys650Glu (c.1948A>G) (PubMed=11157491; Cosmic-CLP).

Heterozygous for SMAD2 p.Leu87Arg (c.260T>G) (Cosmic-CLP).

Homozygous for TP53 p.Arg175His (c.524G>A) (PubMed=21173094; Cosmic-CLP).

HLA信息

Class I

HLA-A        A*24

HLA-B        B*07,15

HLA-C        C*04,07

STR信息

Amelogenin        X

CSF1PO        12,13

D3S1358        15,18

D5S818        13

D7S820        12

D8S1179        10,13

D13S317        11

D16S539        9,13

D18S51        13,14

D21S11        30,33.2

FGA        20,21

Penta D        9

Penta E        12,21

TH01        6,7

TPOX        8

vWA        14,17

參考文獻

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Whole-exon sequencing of human myeloma cell lines shows mutations related to myeloma patients at relapse with major hits in the DNA regulation and repair pathways.

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The LL-100 panel: 100 cell lines for blood cancer studies.

Sci. Rep. 9:8218-8218(2019)

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Cell 180:387-402(2020)